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Polymerase Chain Reaction: A New Era in Detection of Periodontopathogens
Parul Aneja1, Pradeep Shukla2, MSM Biir3, Suruchi Hans4, Vikas Aneja5, Amrit Padda6
1 Senior Lecturer, Department of Periodontics, Maharaja Ganga Singh Dental College, Sriganganagar, India
2 Professor and Head, Department of Periodontics, D.J College of Dental Sciences and Research, Modinagar, India
3 Professor and Head, Department of Periodontics, Maharaja Ganga Singh Dental College, Sriganganagar, India
4 Reader, Department of Periodontics, Maharaja Ganga Singh Dental College, Sriganganagar, India
5 Reader, Department of Oral and Maxillofacial Surgery, Maharaja Ganga Singh Dental College, Sriganganagar, India
6 Senior Lecturer, Department of Periodontics, Surendera Dental College and Hospital, Sriganganagar, India
Correspondence Address:
Parul Aneja
Senior Lecturer, Department of Periodontics, Maharaja Ganga Singh Dental College, Sriganganagar
India
 Source of Support: None, Conflict of Interest: None  | Check |
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Aim: Various techniques like cultural methods, Immunofluorescence and ELISA have largely been used for studies of microbial ecology. But nowdays PCR is used as a diagnostic tool that can detect even small numbers of periodontal pathogens with a high degree of accuracy. In this study, the quantity of periodontal pathogens (P.gingivalis, T.denticola, T.forsythia and A.actinomycetemcomitans) in saliva, preoperatively and postoperatively after scaling and root planing was compared by using multiplex PCR test.
Materials and Method : Unstimulated saliva of 30 chronic periodontitis patients was taken preoperatively by spitting method in eppendorf vial. After treatment i.e scaling and root planing again saliva of the same patient was taken and sent to the laboratory of Maratha Mandal Dental College, Department of Microbiology, Belgaum, Karnataka for PCR analysis.
Results : Quantity of three bacteria (T.d, A.a, P.g) were reduced significantly after scaling and root planing as compared to T.f which did not show statistically significant reduction.
Conclusion : Non-surgical therapy like scaling and root planing plays a very important part in treating the patients of chronic periodontitis. |
